Updated · The Transmitter: Neuroscience News and Perspectives · Aug 12
FlatMux Images Neurons Across 2 Depths at Once, Expanding Beyond 50×250 μm GEVI Limits
Updated
Updated · The Transmitter: Neuroscience News and Perspectives · Aug 12
FlatMux Images Neurons Across 2 Depths at Once, Expanding Beyond 50×250 μm GEVI Limits
1 articles · Updated · The Transmitter: Neuroscience News and Perspectives · Aug 12
Summary
FlatMux lets researchers record neuronal electrical activity across wider brain areas, deeper tissue and multiple depths simultaneously, extending two-photon voltage imaging beyond the small fields that previously captured only about 10 neurons.
1,000-frames-per-second scanning has made GEVI imaging exceptionally difficult because voltage signals are weak, membrane-bound and last only about 1 millisecond, while microscopes must avoid tissue heating and photobleaching.
One pulse per pixel is central to the platform’s design: it balances energy, time and spatial sampling so scan points are dense enough to resolve fine structures without wasting light or burning out sensors.
Nature Methods published the work last month, and outside researchers said the system is a highly optimized advance for two-photon voltage imaging, which could sharpen timing measurements that calcium indicators often blur.